Development of O Antigen Rapid Typing by MALDI-TOF MS

نویسندگان

چکیده

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which provides high sensitivity and resolution, has been used for the analysis of biopolymers. In past decade, MALDI-TOF MS utilized in clinical fields around world to identify microbial species. However, serotype or strain discrimination same bacterial species is difficult by MS. Different methods such as PCR immunoassay are performed identification. these assays required previous preparation selection each probe every candidate, extremely complicated. This study developed a method measure different O antigens from single colony E. coli within one hour. From mixtures strains with antigens, can be assigned without column HILIC manipulation. antigen (serotype) identification method, combined conventional MALDI biotyping (species) toward ribosomal protein, would powerful technique microorganism

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ژورنال

عنوان ژورنال: Journal of the Mass Spectrometry Society of Japan

سال: 2022

ISSN: ['1340-8097', '1880-4225']

DOI: https://doi.org/10.5702/massspec.s22-60